About Next-Gen Sequencing

The first-generation Sanger sequencing method has inherent technical limitations including low-throughput, slow speed, high cost, and difficulty to analyze allele frequency. To achieve the goal of $1,000 human genome, new sequencing technologies have been coming out since 2004. These technologies are massively parallel, thereby achieving high-throughput. The streamlined sample prep step prior to sequencing leads to significant savings in time and cost. Since each sequencing reaction is carried out on one piece of DNA, different alleles can be analyzed at the same time.

 

Key steps of the sequencing-by-synthesis-based Illumina sequencing procedure


CMADP Events

KU Bioengineering Colloquium
co-sponsored by COBRE CMADP

Daniel Citterio, Ph.D.
Professor of Applied Chemistry
Keio University, Yokohama, Japan

"Low-Cost Analytical Devices Made from Porous Substrates: Simple is the Best"
Friday, February 28, 2020 at 2:00pm
Learned Hall, Room 3150

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