About Next-Gen Sequencing

The first-generation Sanger sequencing method has inherent technical limitations including low-throughput, slow speed, high cost, and difficulty to analyze allele frequency. To achieve the goal of $1,000 human genome, new sequencing technologies have been coming out since 2004. These technologies are massively parallel, thereby achieving high-throughput. The streamlined sample prep step prior to sequencing leads to significant savings in time and cost. Since each sequencing reaction is carried out on one piece of DNA, different alleles can be analyzed at the same time.

 

Key steps of the sequencing-by-synthesis-based Illumina sequencing procedure


CMADP Events

Ralph N. Adams Lectureship, KU Chemistry Dept.

R. Mark Wightman, Ph.D.
Professor Emeritus of Chemistry
University of North Carolina, Chapel Hill

Public Lecture "Detecting Catecholamines--A Journey from Beaker to the Behaving Brain"
Thursday, November 15 @ 4:00pm
Integrated Science Building, Room 1154 (Central District)
Reception immediately following lecture in ISB atrium, open to all

Scientific Lecture "Chemical Monitoring of Neurotransmission with Microelectrodes"
Friday, November 16 @ 4:00pm
Integrated Science Building, Room 1154 (Central District)

KU Today