User-accessible Equipment and Sequencing Systems Available

For all user accessible equipment in the Genome Sequencing Core, a monthly fee of $25.00 will be charged to each lab group.


 

Agilent TapeStation for automated DNA/RNA sample and library analysis

Consumables available for purchase at $5.00 per sample.

Alternatively, user can purchase Agilent TapeStation consumables at www.agilent.com.

Please email Jenny Hackett at jhackett@ku.edu for training or to reserve a day and time to use machine.

TapeStation consumables available for use:

DNA Analysis 

  • genomic DNA ​(Part numbers : 5067-5365, 5067-5366)
    • size range: 200bp to >60000bp 
    • quantitative range: 10-100ng/uL 
    • requires 1uL of sample 
  • High Sensitivity D1000 ​(Part numbers : 5067-5584, 5190-6504)
    • size range: 35-1000 bp 
    • quantitative range: 10-1000 pg/uL 
    • requires 2uL of sample 
  • D1000 ​(Part numbers : 5067-5582, 5190-6502)
    • size range: 35-1000 bp 
    • quantitative range: 0.1-50 ng/uL 
    • requires 1uL of sample 

​RNA Analysis

  • High Sensitivity RNA ​(Part numbers : 5067-5579, 5067-5580)
    • size range: 100-6000 bp 
    • quantitative range: 500-10000 pg/uL,
    • requires 2uL of sample 
  • RNA ​(Part numbers : 5067-5576, 5067-5577)
    • size range: 100-6000 bp 
    • quantitative range: 25-500 ng/uL,
    • requires 1uL of sample ​

Blue Pippin for DNA size selection

Consumables available for purchase at $12.00 per lane (5 lanes per cassette).

Alternatively, user can purchase Blue Pippin gel cassettes at www.sagescience.com (Part numbers : BDF1510, BDF2010).

BluePippin gel cassettes come in a variety of % for different size selection ranges:

  • 3% agarose, 90-200bp
  • 2% agarose, 100-600bp
  • 1.5% agarose, 250bp-1.5kb
  • 0.75% agarose, 1-50kb

Please email Jenny Hackett at jhackett@ku.edu for training or to reserve a day and time to use machine.

Blue Pippin Consumables available for use

2% Gel Cassette - allows for size selection in 100-600 bp range, uses internal standards (Part number : BDF2010)


 

Covaris S220 for ultrasound DNA fragmentation

Consumables are available for purchase at $6.00 per sample.

Alternatively, user can purchase Covaris S220 microtubes at www.covarisinc.com (Part number : 520045).

Please email Jenny Hackett at jhackett@ku.edu for training or to reserve a day and time to use machine.

Covaris S220 Consumables available for use

microTUBE AFA Fiber Pre-Slit Snap-Cap 6x16mm - allows for fragmentation of 50 uL DNA samples (Part Number : 520045)


 

BioRad QX200 Digital Droplet PCR System for quantifying DNA molecules

User must purchase consumables for digital droplet PCR, BioRad QX200 Droplet Generator, and BioRad QX200 Droplet Reader.

Please email Jenny Hackett at jhackett@ku.edu for additional information and to reserve a day and time to use machine.

Required consumables and accessories include:

  • ddPCR Supermix Kit
  • DG8 Cartridges and DG8 Gaskets
  • Droplet Generation Oil
  • 96 well plate
  • Multichannel pipet and pipet tips (recommended)
  • Pierceable Foil Heat Seal

Click here to download a pdf of required consumables and part numbers, reagents can be purchased from www.bio-rad.com


 

Illumina Eco qPCR System for library quantification

​Eco qPCR machine is not user accessible at this time, but samples can be qPCR quantified by GSC staff at $20.00 per sample.

Please email Jenny Hackett at jhackett@ku.edu for further information.


 

Illumina MiSeq System

Sequencing Modes Available

  • V2 reagents 
    • 15 million reads per flow cell
    • Runs up to SR-250 or PE-250
  • V3 reagents 
    • 25 million reads per flow cell
    • Runs up to SR-300 or PE-300

​For detailed list of services and pricing, click here.

For more information about Illumina MiSeq system, click here.


 

Illumina HiSeq 2500 System

Sequencing Modes Available

  • High Output - produces 180-200 million reads per lane - 8 lanes per flow cell
    • SR-50, PE-50
    • SR-100, PE-100
  • Rapid Run ​- produces 120-150 million reads per lane - 2 lanes per flow cell - must purchase both lanes of Rapid flow cell
    • SR-50, PE-50
    • SR-100, PE-100
    • SR-150, PE-150
    • SR-200, PE-200
    • SR-250, PE-250

​For detailed list of services and pricing, click here.

For more information about Illumina HiSeq 2500 system, click here.

To monitor sequencing queue status, click here.


 


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