Sample Submission Guidelines

 

gDNA for DNAseq Library Construction

GSC requirements for genomic DNA (gDNA) submission:

  • We recommend that gDNA be submitted at a concentration of 20 ng/µL in 60 µL total
    • 5 µL will be used for sample QC, 55 µL will be used for library prep  ​
  • gDNA should be RNase treated prior to submission and the quality verified by electrophoresis.  
  • We recommend that you quantify gDNA with Qubit
  • DNA should be submitted in clearly labeled 1.7 mL tubes, please limit sample names to 8 characters.

 

Total RNA for RNAseq Library Construction

GSC requirements for total RNA submission:

  • We recommend that total RNA be submitted at a concentration of 20 ng/µL in 60 µL total 
    • ​​10 µL will be used for sample QC, 50 µL will be used for library prep 
  • We recommend that total RNA be Trizol extracted and treated with DNase prior to submission
  • We recommend that you quantify RNA with Qubit
  • RNA should be submitted in clearly labeled 1.7 mL tubes, please limit sample names to 8 characters.
  • We strongly recommend RNA to be stored on dry ice or at -80°C until submission.

 

Investigator Prepared Libraries

The GSC requires that investigator prepared libraries are submitted as follows:

  • Libraries must be prepared according to standard commercially available library kits (eg. Illumina TruSeq, Nextera, NEBNext, etc).
  • Please contact the GSC if you plan to submit custom libraries.
  • Libraries are submitted as 30 µL aliquots at a concentration of ~10 nM.
    • To dilute samples to 10 nM, use nM conversion calculator, this will give an estimated nM concentration
    • Use Qubit quantification to get ng/µL concentration and TapeStation/Bioanalyzer gel analysis for average bp size estimation
  • Accurate quantification of libraries is essential for flow cell clustering. We strongly recommend that libraries are quantified by qPCR. The GSC can perform qPCR of a sample for a fee.
  • Please submit these samples in a clearly labeled 1.7 mL tube; please limit the sample names to 8 characters.

 

Sample Drop Off

Samples can be delivered to the GSC Monday through Friday, between 9:00 AM and 6:00 PM.

Samples can also be mailed to the GSC on dry ice at this address:

          Genome Sequencing Core

          University of Kansas

          1030 Haworth Hall

          1200 Sunnyside Avenue

          Lawrence KS 66045

 

 

 

 


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