Sample Submission Guidelines

 

gDNA for DNAseq Library Construction

GSC requirements for genomic DNA (gDNA) submission:

  • We recommend that gDNA be submitted at a concentration of 20 ng/µL in 60 µL total
    • 5 µL will be used for sample QC, 55 µL will be used for library prep  ​
  • gDNA should be RNase treated prior to submission and the quality verified by electrophoresis.  
  • We recommend that you quantify gDNA with Qubit
  • DNA should be submitted in clearly labeled 1.7 mL tubes, please limit sample names to 8 characters.

 

Total RNA for RNAseq Library Construction

GSC requirements for total RNA submission:

  • We recommend that total RNA be submitted at a concentration of 20 ng/µL in 60 µL total 
    • ​​10 µL will be used for sample QC, 50 µL will be used for library prep 
  • We recommend that total RNA be Trizol extracted and treated with DNase prior to submission
  • We recommend that you quantify RNA with Qubit
  • RNA should be submitted in clearly labeled 1.7 mL tubes, please limit sample names to 8 characters.
  • We strongly recommend RNA to be stored on dry ice or at -80°C until submission.

 

Investigator Prepared Libraries

The GSC requires that investigator prepared libraries are submitted as follows:

  • Libraries must be prepared according to standard commercially available library kits (eg. Illumina TruSeq, Nextera, NEBNext, etc).
  • Please contact the GSC if you plan to submit custom libraries.
  • Libraries are submitted as 30 µL aliquots at a concentration of ~10 nM.
    • To dilute samples to 10 nM, use nM conversion calculator, this will give an estimated nM concentration
    • Use Qubit quantification to get ng/µL concentration and TapeStation/Bioanalyzer gel analysis for average bp size estimation
  • Accurate quantification of libraries is essential for flow cell clustering. We strongly recommend that libraries are quantified by qPCR. The GSC can perform qPCR of a sample for a fee.
  • Please submit these samples in a clearly labeled 1.7 mL tube; please limit the sample names to 8 characters.

 

Sample Drop Off

Samples can be delivered to the GSC Monday through Friday, between 9:00 AM and 6:00 PM.

Samples can also be mailed to the GSC on dry ice at this address:

          Genome Sequencing Core

          University of Kansas

          1030 Haworth Hall

          1200 Sunnyside Avenue

          Lawrence KS 66045

 

 

 

 


CMADP Events

Special seminar by Dr. Kevin W. Plaxco
Professor of Chemistry & Biochemistry
UC Santa Barbara

Wednesday, April 19, 2017 at 4:00pm
School of Pharmacy, Room 3020

"Counting molecules, dodging blood cells: real-time molecular measurements directly in the living body"
The development of technology capable of continuously tracking the levels of drugs, metabolites, and biomarkers in situ in the body would revolutionize our understanding of health and our ability to detect and treat disease. It would, for example, provide clinicians with a real-time window into organ function and would enable therapies guided by patient-specific, real-time pharmacokinetics, opening a new dimension in personalized medicine. In response my group has pioneered the development of a “biology-inspired” electrochemical approach to monitoring specific molecules that supports real-time measurements of arbitrary molecular targets (irrespective of their chemical reactivity) directly in awake, fully ambulatory subjects.
KU Today