ddPCR Usage
The ddPCR system is available for user operation after initial training. Consumables are available to purchase from the GSC.
Users are required to design an experiment, design primers or probes and prepare the appropriate Supermix reaction - according to the Probes or EvaGreen protocols (available on the Bio-Rad website) prior to using the ddPCR system.
Users will need to purchase the ddPCR Supermix Kit appropriate for either the Probes or EvaGreen protocol. Supermix kits are available for purchase from the Bio-Rad website.
Prior to using the GSC ddPCR system, users will prepare the Supermix reaction and bring this ~20-22ul reaction volume to the GSC ready to generate droplets. Supermix reactions are stable for 24 hours at room temperature.
ddPCR Consumables Stocked by the GSC (and available for use with GSC equipment)
DG8 Cartridges and DG8 Gaskets
Droplet Generation Oil (EvaGreen/Probes)
BioRad QX200 96 well plate
Rainin multichannel pipet and pipet tips
Pierceable Foil Heat Seal
​Droplet Reader Oil
Users can also purchase ddPCR consumables on the Bio-Rad website.
ddPCR Sign-up
Please email us at gsc@ku.edu for training or to set up a time to drop off samples for ddPCR analysis.
To sign-up to use the ddPCR, you must submit an email request to gsc@ku.edu.
Or after completing training, sign up to use the ddPCR on the ddPCR Calendar (Google calendar).
On the ddPCR schedule, please include:
Your Name
PI's Name
Reservation Time
Estimated number of samples