Services and Pricing

Users can download the Illumina Sequencing Coverage Calculator to estimate the number of reads required for your experiment.

The GSC does not offer Sanger or Plasmid Sequencing as part of its services.

For Sanger Sequencing services, the BioStore (Haworth 3027) has a dropbox for ACGT.

For Plasmid Sequencing services, the BioStore (Haworth 3027) has a drop box for PlasmidSaurus.

If you have any questions, please contact the BioStore for more information.

DNA/RNA Submission Form (Excel document)

Library Pool Submission Form (Excel document)

Library Sample Submission Form (Excel document)

• We recommend that gDNA be submitted at a concentration of 20 ng/µL in 35 µL total - if gDNA is quantified by Nanodrop, please submit at a concentration of 40 ng/µL in 35 µL total
  • 5 µL will be used for sample QC, 30 µL will be used for library prep  ​
  • Library prep kit uses an input of 100-500 ng in 30 µL
• gDNA should be RNase treated prior to submission and the quality verified by electrophoresis.  
• We recommend that you quantify gDNA with Qubit
• DNA should be submitted in clearly labeled 1.7 mL tubes, please limit sample names to 8 characters.
• Sample names must use only alpha numeric characters and "-" or "_" - dashes or underscores
  • Please do not include " . ) ( / \ } { ] [ ? = + > < : ; " ' * ^ | & " - periods, parentheses, back or forward slashes, brackets, question mark, equals sign, addition sign, chevrons, colon, semicolon, quote sign, apostrophe, asterisk, carrot, bar or ampersand.

• We recommend that total RNA be submitted at a concentration of 20 ng/µL in 60 µL total - if RNA is quantified by Nanodrop, please submit at a concentration of 40 ng/µL in 60 µL total
  • ​​10 µL will be used for sample QC, 50 µL will be used for library prep 
  • Library prep kit uses an input of 10-1000 ng in 50 µL
• We recommend that total RNA be Trizol extracted and treated with DNase prior to submission
• We recommend that you quantify RNA with Qubit
• We strongly recommend RNA to be stored on dry ice or at -80ºC until submission
• RNA should be submitted in clearly labeled 1.7 mL tubes, please limit sample names to 8 characters.
• Sample names must use only alphanumeric characters and "-" or "_" - dashes or underscores
  • Please do not include " . ) ( / \ } { ] [ ? = + > < : ; " ' * ^ | & " - periods, parentheses, back or forward slashes, brackets, question mark, equal sign, addition sign, chevrons, colon, semicolon, quote sign, apostrophe, asterisk, carrot, bar or ampersand.

• Libraries must be prepared according to standard commercially available library kits (eg. Illumina TruSeq, Nextera, NEBNext, etc).
• Please contact the GSC if you plan to submit custom libraries.
• Libraries are submitted as 30 µL aliquots at a concentration of ~10 nM.
  • To dilute samples to 10 nM, use the nM conversion calculator (Excel document), this will give an estimated nM concentration
  • Use Qubit quantification to get ng/µL concentration and TapeStation/Bioanalyzer gel analysis for average bp size estimation
• Accurate quantification of libraries is essential for flow cell clustering. We strongly recommend that libraries are quantified by qPCR. The GSC can perform qPCR for a fee.
• Please submit these samples in a clearly labeled 1.7 mL tube; please limit the sample names to 8 characters.
• Sample names must use only alphanumeric characters and "-" or "_" - dashes or underscores
  • Please do not include " . ) ( / \ } { ] [ ? = + > < : ; " ' * ^ | & " - periods, parentheses, back or forward slashes, brackets, question mark, equal sign, addition sign, chevrons, colon, semicolon, quote sign, apostrophe, asterisk, carrot, bar or ampersand.

Use the Sequencing Queue Calendar to monitor the status of a sequencing run

"MS" = MiSeq, "NX" = NextSeq550, "NX2K" = NextSeq2000

Data from finished runs will typically be processed the following business day.

This is an anticipated schedule and is subject to changes at short notice.